Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64A

Authors

Lisa A. Fitzgerald, University of Nebraska-LincolnFollow
Michael V. Graves, University of Massachusetts–LowellFollow
Xiao Li, University of Massachusetts–Lowell
Tamara Feldblyum, The Institute for Genomic Research, Rockville, MD
Willaim C. Nierman, The Institute for Genomic Research, Rockville, MDFollow
James L. Van Etten, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

2-20-2007

Comments

Published in Virology 358:2 (February 20, 2007), pp. 472-484. doi:10.1016/j.virol.2006.08.033 Copyright © 2006 Elsevier Inc. Used by permission. http://www.sciencedirect.com/science/journal/00426822

Abstract

Viruses NY-2A and AR158, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella NC64A. The 368,683-bp genome of NY-2A and the 344,690-bp genome of AR158 are the two largest chlorella virus genomes sequenced to date; NY-2A contains 404 putative protein-encoding and 7 tRNA-encoding genes and AR158 contains 360 putative protein-encoding and 6 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Two of the NY-2A genes encode inteins, the large subunit of ribonucleotide reductase and a superfamily II helicase. These are the first inteins to be detected in the chlorella viruses. Approximately 40% of the viral gene products resemble entries in the public databases, including some that are unexpected for a virus. These include GDP-d-mannose dehydratase, fucose synthase, aspartate transcarbamylase, Ca⁺⁺ transporting ATPase and ubiquitin. Comparison of NY-2A and AR158 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that 85% of the genes are present in all three viruses.

Supplementary data for this article is posted in this repository in 4 separate files.