Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64A

Authors

• Lisa A. Fitzgerald, University of Nebraska-LincolnFollow
• Michael V. Graves, University of Massachusetts–LowellFollow
• Xiao Li, University of Massachusetts–Lowell
• Tamara Feldblyum, The Institute for Genomic Research, Rockville, MD
• Willaim C. Nierman, The Institute for Genomic Research, Rockville, MDFollow
• James L. Van Etten, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

2-20-2007

Comments

Published in Virology 358:2 (February 20, 2007), pp. 472-484. doi:10.1016/j.virol.2006.08.033 Copyright © 2006 Elsevier Inc. Used by permission. http://www.sciencedirect.com/science/journal/00426822

Abstract

Viruses NY-2A and AR158, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella NC64A. The 368,683-bp genome of NY-2A and the 344,690-bp genome of AR158 are the two largest chlorella virus genomes sequenced to date; NY-2A contains 404 putative protein-encoding and 7 tRNA-encoding genes and AR158 contains 360 putative protein-encoding and 6 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Two of the NY-2A genes encode inteins, the large subunit of ribonucleotide reductase and a superfamily II helicase. These are the first inteins to be detected in the chlorella viruses. Approximately 40% of the viral gene products resemble entries in the public databases, including some that are unexpected for a virus. These include GDP-d-mannose dehydratase, fucose synthase, aspartate transcarbamylase, Ca⁺⁺ transporting ATPase and ubiquitin. Comparison of NY-2A and AR158 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that 85% of the genes are present in all three viruses.

Supplementary data for this article is posted in this repository in 4 separate files.