Production of Acquired Immunodeficiency Syndrome-Associated Retrovirus in Human and Nonhuman Cells Transfected with an Infectious Molecular Clone

Authors

• Akio Adachi, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland
• Howard E. Gendelman, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland
• Scott Koenig, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland
• Thomas Folks, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland
• Ronald Willey, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland
• Arnold Rabson, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland
• Malcom A. Martin, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

Document Type

Article

Date of this Version

August 1986

Comments

Published in JOURNAL OF VIROLOGY, Aug. 1986, p. 284-291 0022-538X/86/080284-08$02.00/0. Copyright © 1986, American Society for Microbiology Vol. 59. No. 2. Used by permission.

Abstract

We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.