Virology, Nebraska Center for


Date of this Version

August 1986


Published in JOURNAL OF VIROLOGY, Aug. 1986, p. 284-291 0022-538X/86/080284-08$02.00/0. Copyright © 1986, American Society for Microbiology Vol. 59. No. 2. Used by permission.


We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.

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