Expression of functional protease and subviral particles by vaccinia virus containing equine infectious anaemia virus gag and 5' pol genes

Authors

Travis C. McGuire, Washington State University
Katherine I. O'Rourke, U.S. Department of AgricultureFollow
Timothy V. Baszler, Washington State University
Steven R. Leib, Washington State University
Alberta L. Brassfield, Washington State University
William C. Davis, Washington State UniversityFollow

Document Type

Article

Date of this Version

1994

Comments

Published in Journal of General Virology (1994), 75, 895-900

Abstract

Cells infected with vaccinia viruses expressing the equine infectious anaemia virus (EIAV) gag gene (VGag) or gag plus the 5' pol encoding protease (VGag/PR) were evaluated with monoclonal antibody to a p26 capsid protein linear epitope (QEISKFLTD). Both recombinant viruses expressed Gag precursor protein (55K) whereas only VGag/PR expressed a detectable Gag-Pol fusion protein (82K) with a functional protease, shown by subviral particles containing processed p26. Horses inoculated with VGag/PR produced antibodies reactive with EIAV Gag proteins.