Expression of functional protease and subviral particles by vaccinia virus containing equine infectious anaemia virus gag and 5' pol genes

Authors

• Travis C. McGuire, Washington State University
• Katherine I. O'Rourke, U.S. Department of AgricultureFollow
• Timothy V. Baszler, Washington State University
• Steven R. Leib, Washington State University
• Alberta L. Brassfield, Washington State University
• William C. Davis, Washington State UniversityFollow

Document Type

Article

Date of this Version

1994

Comments

Published in Journal of General Virology (1994), 75, 895-900

Abstract

Cells infected with vaccinia viruses expressing the equine infectious anaemia virus (EIAV) gag gene (VGag) or gag plus the 5' pol encoding protease (VGag/PR) were evaluated with monoclonal antibody to a p26 capsid protein linear epitope (QEISKFLTD). Both recombinant viruses expressed Gag precursor protein (55K) whereas only VGag/PR expressed a detectable Gag-Pol fusion protein (82K) with a functional protease, shown by subviral particles containing processed p26. Horses inoculated with VGag/PR produced antibodies reactive with EIAV Gag proteins.