Validation of monoclonal antibody F99/97.6.1 for immunohistochemical staining of brain and tonsil in mule deer (Odocoileus hemionus) with chronic wasting disease

Authors

T. R. Spraker, Colorado State University - Fort Collins
Katherine I. O'Rourke, U.S. Department of AgricultureFollow
A. Balachandran, Animal Disease Research Inspection
R. R. Zink, Colorado State University - Fort Collins
B. A. Cummings, Colorado State University - Fort Collins
M. W. Miller, Colorado Division of WildlifeFollow
B. E. Powers, Colorado State University - Fort Collins

Document Type

Article

Date of this Version

2002

Comments

Published in J Vet Diagn Invest 14:3–7 (2002)

Abstract

A new monoclonal antibody (MAb), F99/97.6.1, that has been used to demonstrate scrapieassociated prion protein PrP^Sc in brain and lymphoid tissues of domestic sheep with scrapie was used in an immunohistochemistry assay for diagnosis of chronic wasting disease (CWD) in mule deer (Odocoileus hemionus). The MAb F99/97.6.1 immunohistochemistry assay was evaluated in brain and tonsil tissue from 100 mule deer that had spongiform encephalopathy compatible with CWD and from 1,050 mule deer outside the CWD-endemic area. This MAb demonstrated abnormal protease-resistant prion protein (PrP^res) in brains of all of the 100 mule deer and in 99 of the 100 tonsil samples. No immunostaining was seen in samples collected from deer outside the endemic area. MAb F99/97.6.1 demonstrated excellent properties for detection of PrP^res in fresh, frozen, or mildly to moderately autolytic samples of brain and tonsil. This immunohistochemistry assay is a sensitive, specific, readily standardized diagnostic test for CWD in deer.