descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques

Authors

Kohei Otomo, Juntendo University Graduate School of Medicine, JuntendoUniversity School of Medicine, National Institutes of Natural Sciences
Takaki Omura, Juntendo University Graduate School of Medicine, University of Tokyo, National Cancer Center Hospital
Yuki Nozawa, JuntendoUniversity School of Medicine
Steven J. Edwards, KTH Royal Institute of Technology
Yukihiko Sato, Juntendo University Graduate School of Medicine
Yuri Saito, Juntendo University Graduate School of Medicine
Shigehiro Yagishita, National Cancer Center Research Institute
Hitoshi Uchida, Niigata University
Yuki Watakabe, National Institutes of Natural Sciences
Kiyotada Naitou, Kagoshima University
Rin Yanai, National Institutes for Quantum Science and Technology
Naruhiko Sahara, National Institutes for Quantum Science and Technology
Satoshi Takagi, Japanese Foundation for Cancer Research
Ryohei Katayama, Japanese Foundation for Cancer Research
Yusuke Iwata, The University of Tokyo
Toshiro Shiokawa, The University of Tokyo
Yoku Hayakawa, The University of Tokyo
Kensuke Otsuka, Central Research Institute of Electric Power Industry
Haruko Watanabe-Takano, Nippon Medical School
Yuka Haneda, Nippon Medical School
Shigetomo Fukuhara, Nippon Medical School
Miku Fujiwara, Kyushu University
Takenobu Nii, Kyushu University
Chikara Meno, Kyushu University
Naoki Takeshita, Kyoto Prefectural University of Medicine
Kenta Yashiro, Kyoto Prefectural University of Medicine
Juan Marcelo Rosales Rocabado, Niigata University
Masaru Kaku, Niigata University
Tatsuya Yamada, University of Nebraska-Lincoln
Yumiko Oishi, Tokyo Medical and Dental University
Hiroyuki Koike, Tokyo Medical and Dental University
Yinglan Cheng, Tokyo Medical and Dental University
Keisuke Sekine, National Cancer Center Research Institute
Jun-ichiro Koga, University of Occupational and Environmental Health
Kaori Sugiyama, Waseda University
Kenichi Kimura, University of Tsukuba
Fuyuki Karube, Hokkaido University
Hyeree Kim, Chiba University
Ichiro Manabe, Chiba University
Tomomi Nemoto, National Institutes of Natural Sciences
Kazuki Tainaka, Niigata University
Akinobu Hamada, National Cancer Center Research Institute
Hjalmar Brismar, KTH Royal Institute of Technology
Etsuo A. Susaki, Juntendo University Graduate School of Medicine, JuntendoUniversity School of Medicine

Document Type

Article

Date of this Version

5-24-2024

Citation

Nature Communications | ( 2024) 15:4941. https://doi.org/10.1038/s41467-024-49131-1

Comments

Open access.

Abstract

Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktopequipped SPIM for cleared specimens), a low-cost ($20,000–50,000), lowexpertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a threedimensional manner. Academically open-sourced (https://github.com/dbsbjuntendo/ descSPIM), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.