descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques

Authors

• Kohei Otomo, Juntendo University Graduate School of Medicine, JuntendoUniversity School of Medicine, National Institutes of Natural Sciences
• Takaki Omura, Juntendo University Graduate School of Medicine, University of Tokyo, National Cancer Center Hospital
• Yuki Nozawa, JuntendoUniversity School of Medicine
• Steven J. Edwards, KTH Royal Institute of Technology
• Yukihiko Sato, Juntendo University Graduate School of Medicine
• Yuri Saito, Juntendo University Graduate School of Medicine
• Shigehiro Yagishita, National Cancer Center Research Institute
• Hitoshi Uchida, Niigata University
• Yuki Watakabe, National Institutes of Natural Sciences
• Kiyotada Naitou, Kagoshima University
• Rin Yanai, National Institutes for Quantum Science and Technology
• Naruhiko Sahara, National Institutes for Quantum Science and Technology
• Satoshi Takagi, Japanese Foundation for Cancer Research
• Ryohei Katayama, Japanese Foundation for Cancer Research
• Yusuke Iwata, The University of Tokyo
• Toshiro Shiokawa, The University of Tokyo
• Yoku Hayakawa, The University of Tokyo
• Kensuke Otsuka, Central Research Institute of Electric Power Industry
• Haruko Watanabe-Takano, Nippon Medical School
• Yuka Haneda, Nippon Medical School
• Shigetomo Fukuhara, Nippon Medical School
• Miku Fujiwara, Kyushu University
• Takenobu Nii, Kyushu University
• Chikara Meno, Kyushu University
• Naoki Takeshita, Kyoto Prefectural University of Medicine
• Kenta Yashiro, Kyoto Prefectural University of Medicine
• Juan Marcelo Rosales Rocabado, Niigata University
• Masaru Kaku, Niigata University
• Tatsuya Yamada, University of Nebraska-Lincoln
• Yumiko Oishi, Tokyo Medical and Dental University
• Hiroyuki Koike, Tokyo Medical and Dental University
• Yinglan Cheng, Tokyo Medical and Dental University
• Keisuke Sekine, National Cancer Center Research Institute
• Jun-ichiro Koga, University of Occupational and Environmental Health
• Kaori Sugiyama, Waseda University
• Kenichi Kimura, University of Tsukuba
• Fuyuki Karube, Hokkaido University
• Hyeree Kim, Chiba University
• Ichiro Manabe, Chiba University
• Tomomi Nemoto, National Institutes of Natural Sciences
• Kazuki Tainaka, Niigata University
• Akinobu Hamada, National Cancer Center Research Institute
• Hjalmar Brismar, KTH Royal Institute of Technology
• Etsuo A. Susaki, Juntendo University Graduate School of Medicine, JuntendoUniversity School of Medicine

Document Type

Article

Date of this Version

5-24-2024

Citation

Nature Communications | ( 2024) 15:4941. https://doi.org/10.1038/s41467-024-49131-1

Comments

Open access.

Abstract

Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktopequipped SPIM for cleared specimens), a low-cost ($20,000–50,000), lowexpertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a threedimensional manner. Academically open-sourced (https://github.com/dbsbjuntendo/ descSPIM), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.