Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

Authors

Qamar Taban, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, University of Kashmir
Syed Mudasir Ahmad, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir
Peerzada Tajamul Mumtaz, University of Nebraska-LincolnFollow
Basharat Bhat, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir
Ehtishamul Haq, University of Kashmir
Suhail Magray, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir
Sahar Saleem, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir
Nadeem Shabir, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir
Amatul Muhee, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir
Zahid Amin Kashoo, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir
Mahrukh Hameed Zargar6, Sher-I-Kashmir Institute of Medical Sciences
Abrar A. Malik, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir
Nazir A. Ganai, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir
Riaz A. Shah, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir

Date of this Version

1-5-2023

Citation

Taban et al. Cell Communication and Signaling (2023) 21:3 https://doi.org/10.1186/s12964-022-01014-y

Comments

Open access.

Abstract

SCARB1 belongs to class B of Scavenger receptors (SRs) that are known to be involved in binding and endocytosis of various pathogens. SRs have emerging role in regulating innate immunity and host–pathogen interactions by acting in co-ordination with Toll-like receptors.Query Little is known about the function of SCARB1 in milk-derived mammary epithelial cells (MECs). This study reports the role of SCARB1 in infection and its potential association in TLR4 signaling on bacterial challenge in Goat mammary epithelial cells (GMECs). The novelty in the establishment of MEC culture lies in the method that aims to enhance the viability of the cells with intact characteristics upto a higher passage number. We represent MEC culture to be used as a potential infection model for deeper understanding of animal physiology especially around the mammary gland. On E.coli challenge the expression of SCARB1 was signifcant in induced GMECs at 6 h. Endoribonuclease-esiRNA based silencing of SCARB1 affects the expression of TLR4 and its pathways i.e. MyD88 and TRIF pathways on infection. Knockdown also affected the endocytosis of E.coli in GMECs demonstrating that E.coli uses SCARB1 function to gain entry in cells. Furthermore, we predict 3 unique protein structures of uncharacterized SCARB1 (Capra hircus) protein. Overall, we highlight SCARB1 as a main participant in host defence and its function in antibacterial advances to check mammary gland infections.