Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera

Authors

B. S. Coates, USDA-ARSFollow
A. P. Alves, University of Nebraska-Lincoln
H. Wang, University of Nebraska-Lincoln
X. Zhou, University of Nebraska-Lincoln
T. Nowatzki, University of Nebraska-Lincoln
H. Chen, University of Nebraska-Lincoln
M. Rangasamy, University of Nebraska-Lincoln
H. M. Robertson, University of IllinoisFollow
C. W. Whitfield, University of IllinoisFollow
K. K. Walden, University of Illinois
Stephen D. Kachman, University of Nebraska-LincolnFollow
B. W. French, USDA-ARSFollow
L. J. Meinke, University of Nebraska-LincolnFollow
D. Hawthrone, University of MarylandFollow
C. A. Abel, Iowa State UniversityFollow
T. W. Sappington, Iowa State UniversityFollow
B. D. Siegfried, University of Nebraska-LincolnFollow
N. J. Miller, University of Nebraska-LincolnFollow

ORCID IDs

Stephen D. Kachman

N. J. Miller

Date of this Version

2016

Citation

Insect Molecular Biology (2016) 25(1), pp. 1–15, doi: 10.1111/imb.12194.

Comments

U. S. government work.

Abstract

The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in resistant phenotypes, with the most significant (fold increases >/= 2.43) being an a-esterase-like transcript. Differential expression was validated only for the aesterase (ST020027A20C03), with 11- to 13-fold greater expression in methyl-parathion resistant adults (P