U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska
Document Type
Article
Date of this Version
2016
Citation
Insect Molecular Biology (2016) 25(1), pp. 1–15, doi: 10.1111/imb.12194.
Abstract
The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in resistant phenotypes, with the most significant (fold increases >/= 2.43) being an a-esterase-like transcript. Differential expression was validated only for the aesterase (ST020027A20C03), with 11- to 13-fold greater expression in methyl-parathion resistant adults (P
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Comments
U. S. government work.