U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska
Date of this Version
3-2017
Citation
ANNUAL REPORT OF THE BEAN IMPROVEMENT COOPERATIVE, No. 60, March 2017. Published by USDA.
Abstract
INTRODUCTION Seed phosphorus (P) is a fundamental energy source that acts on germination and seedling development. In addition to the inorganic P (Pi), the seed also accumulates as phytate salts and other forms of phosphate compounds denominated organic P (Po). Although necessary for the seed development, an excess of Po may interfere with the accumulation of calcium, iron and zinc nutrients, which are easily complexed with P and become unavailable for human diet and animal feed (RABOY, 2009). The availability of P in the soil or nutrient solution may influence the storage forms of this mineral in the seed. Therefore, the study aimed to evaluate the partition of the absorbed P in common bean genotypes grown in different levels of P in hydroponic solution.
MATERIAL AND METHODS The experiment was carried out in the greenhouse at Agronomic Institute of Paraná (IAPAR), Londrina, Paraná, Brazil, in September to December 2015. The experimental design was a randomized block with six replicates and treatments arranged in a factorial design with two genotypes (BRS Estilo and IPR Tangará) and five P levels (2, 4, 6, 8 e 10 mg L-1). The seedlings with uniform shoot and roots were transplanted into polyethylene pots with capacity 3.35 L containing nutrient solution Hoagland and Arnon (1950), modified by Pavan and Bingham (1982). The redox potential (pH) and electrical conductivity (EC) of the nutritive solution were monitored daily. The solution was aerated continuously and when the electric conductivity reached 0.14 dS cm-2 the nutrient solution was exchanged. To determine total P content the seeds were ground in Perten 3100 mill and submitted to nitroperchloric digestion according to the methodology of Miyazawa et al. (1999). Pi content was extracted according to the method proposed by Raboy and Dickson (1984) and it was quantified by the method described by Chen et al. (1956). Po content was obtained by subtracting the Pi from the total P. The phytate content was determined according to the methodology of Oomah et al. (2008). The data have been subjected to analysis of variance and test Tukey (p ≤ 0.05) for mean comparison. The statistical analyzes were performed using the Sisvar version 5.3 software (FERREIRA, 2010).
Comments
U.S. government work.