U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska
Document Type
Article
Date of this Version
3-2017
Citation
ANNUAL REPORT OF THE BEAN IMPROVEMENT COOPERATIVE, No. 60, March 2017. Published by USDA.
Abstract
Common beans (Phaseolus vulgaris L.) are native from Mexico. The crop has a great economic and social importance and it is a major source of protein and essential nutrients. Drought is the main stress factor on bean production in Mexico and frequently is combined with high incidences of diseases caused by fungi, bacteria, viruses or nematodes. One emerging pathogen in beans and other crops is the fungus Macrophomina phaseolina (Tassi) Goid., causal agent of charcoal rot which incidences are favored by water deficits (Hernández-Delgado et al., 2011; García-Olivares et al., 2012). This work was developed to apply DNA molecular markers to develop a genetic map for identification of molecular markers associated to genes that confer resistance to combined charcoal rot disease/drought stress.
A population of 94 RILs F2:9 from crosses between BAT 477 (resistant to both charcoal rot and water stresses) and cv. Pinto UI-114 (susceptible) was generated. Evaluations of reactions to M. phaseolina and drought stress were conducted under both field and controlled conditions. Controlled evaluations were conducted in Reynosa, México; field experiments were carried out in Rio Bravo, Cotaxtla and Isla, México and were described by García-Olivares et al. (2012). A genetic linkage map was built with genotypic data obtained with 30 +3/+3 AFLP marker combinations which generated 476 polymorphic markers, 190 of them segregating in a 1:1 ratio. Finally, QTLs associated with resistance to both stresses were identified using R ver. 2.10.1 (R Development Core Team, 2012)
Comments
U.S. government work.