Effect of Citrus Byproducts on Survival of O157:H7 and Non-O157 Escherichia coli Serogroups within In Vitro Bovine Ruminal Microbial Fermentations

Authors

Heather A. Duoss-Jennings, Iowa State University
Ty B. Schmidt, University of Nebraska-LincolnFollow
Todd R. Callaway, USDA, Agricultural Research ServiceFollow
Jeffery A. Carroll, USDA, Agricultural Research Service
James M. Martin, Mississippi State University
Sara A. Shields-Menard, Mississippi State University
Paul R. Broadway, Texas Tech University
Janet R. Donaldson, Mississippi State UniversityFollow

Document Type

Article

Date of this Version

2013

Citation

Volume 2013, Article ID 398320, 5 pages

Comments

Copyright © 2013 Heather A. Duoss-Jennings et al.

This is an open access article

http://dx.doi.org/10.1155/2013/398320

Abstract

Citrus byproducts (CBPs) are utilized as a low cost nutritional supplement to the diets of cattle and have been suggested to inhibit the growth of both Escherichia coli O157:H7 and Salmonella. The objective of this study was to examine the effects in vitro that varying concentrations of CBP in the powdered or pelleted variety have on the survival of Shiga-toxin Escherichia coli (STEC) serotypes O26:H11, O103:H8, O111:H8, O145:H28, and O157:H7 in bovine ruminal microorganism media. The O26:H11, O111:H8, O145:H28, and O157:H7 serotypes did not exhibit a change in populations in media supplemented with CBP with either variety. The O103:H8 serotype displayed a general trend for an approximate 1 log₁₀ reduction in 5% powdered CBP and 20% pelleted CBP over 6 h. There was a trend for reductions in populations of a variant form of O157:H7 mutated in the stx1 and stx2 genes in higher concentrations of CBP. These results suggest that variations exist in the survival of these serotypes of STEC within mixed ruminal microorganism fluid media when supplemented with CBP. Further research is needed to determine why CBPs affect STEC serotypes differently.