Western Immunoblotting in Avian Shell Gland Sample Immunoblotting Methods1

Authors

K.K. Hansen, University of Nebraska Medical Center
R.J. Kittok, University of Nebraska-LincolnFollow
Gautam Sarath, University of Nebraska - LincolnFollow
Mary M. Beck, University of Nebraska - LincolnFollow

Date of this Version

2014

Citation

2004 Poultry Science Association, Inc.

Comments

2004 Poultry Science 83:230–233

Abstract

Avian shell gland tissue was subjected to Western blot analysis using anti-human estrogen receptor- α antibody H222. Initial attempts to obtain consistent, high-quality blots were unsuccessful because, as it turned out, excessive lipid in tissue preparations interfered with protein separation. Incremental additions of acetone eventually proved to be the critical step in solubilizing lipids and allowing consistent separation of bands on gels. A detailed description of the methodology is presented.