Animal Science, Department of


Document Type


Date of this Version



Veterinary Clinics of North America: Equine Practice 36:2 (August 2020), pp. 195–209.

doi: 10.1016/j.cveq.2020.03.002


Copyright © 2020 Elsevier, Inc. Used by permission.


  • Next-generation sequencing of both DNA and RNA represents a second revolution in equine genetics following publication of the equine genome sequence.
  • Technological advancements have resulted in a wide selection of next-generation sequencing platforms capable of completing small targeted experiments or resequencing complete genomes.
  • DNA and RNA sequencing have applications in clinical and research environments.
  • Standards for the validation and sharing of next-generation sequencing data are critical for the widespread application of the technology and applications discussed herein.
  • As researchers and clinicians develop a better understanding of how genetic variation and phenotypic variation are linked, next-generation sequencing could help pave the way to personalized and precision management of horses.