Effects over Lambs on Muscle Time of Feeding a β-Adrenergic Agonist to Wether Animal Performance, Muscle Growth, Endogenous Proteinase Activities, and Meat Tenderness

Authors

• T. D. Pringle, University of Nebraska-Lincoln
• Chris R. Calkins, University of Nebraska-LincolnFollow
• Mohammad Koohmaraie, University of Nebraska-LincolnFollow
• Steven J. Jones, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

1993

Comments

Published in Journal of Animal Science 1993. 71:636-644. Copyright © 1993 American Society of Animal Science. Used by permission.

Abstract

Forty wether lambs were used in a 2 x 4 factorial arrangement to determine the response of animal performance, muscle growth, proteinase activity, and meat tenderness to 0-adrenergic agonist (BAA) supplementation. Lambs were fed a finishing diet with or without 4 ppm of L_644,969 and slaughtered after 0, 2, 4, and 6 wk of treatment. The ADG was higher (P < .05) in the treated than in the control lambs after 2 wk and returned to control levels thereafter. Semitendinosus weight and calpastatin activity were higher and μ-calpain activity was lower in the treated than in the control lambs after 2, 4, and 6 wk. Cathepsin B activity was higher ( P < .0 1) and cystatin-like activity was lower ( P < .05) after 2 wk in treated than in control lambs but returned to control levels thereafter. Longissimus protein:DNA was higher after 4 ( P < .05) and 6 ( P < .01) wk in the treated lambs than in the controls. The concentration of RNA and RNA:DNA ratio were higher ( P < .0 1) in the longissimus and semitendinosus muscles in the treated lambs after 2 wk and remained higher throughout the study. Semitendinosus protein and RNA content were higher after 2, 4, and 6 wk and DNA content was higher after 2 and 6 wk in the treated than in the control lambs. Longissimus shearforce values were higher ( P < .001) in the treated than in the control lambs at all slaughter end points. These data indicate a rapid alteration of muscle growth, activity of the calpain-calpastatin system, and meat tenderness during BAA treatment. It also seems that the BAA-stimulated muscle growth is closely related to the activity of the calpain-calpastatin system.