Characterization of three Chlorella sorokiniana strains in anaerobic digested effluent from cattle manure

Authors

Naoko Kobayashi, University of Nebraska-Lincoln
Eric A. Schacht, University of Nebraska-Lincoln
Austin Barnes, University of Nebraska-Lincoln
Andrea K. Watson, University of Nebraska-LincolnFollow
Julian N. Rosenberg, Johns Hopkins University
Galen Erickson, University of Nebraska-LincolnFollow
George A. Oyler, University of Nebraska-Lincoln

ORCID IDs

Andrea K. Watson

Document Type

Article

Date of this Version

2013

Citation

Bioresource Technology 150 (2013) 377–386

Comments

Copyright 2013 The Authors. Open Access Article

Abstract

Chlorella sorokiniana CS-01, UTEX 1230 and UTEX 2714 were maintained in 10% anaerobic digester effluent (ADE) from cattle manure digestion and compared with algal cultivation in Bold’s Basal Medium (BBM). Biomass of CS-01 and UTEX 1230 in ADE produced similar or greater than 280 mg/L after 21 days in BBM, however, UTEX 2714 growth in ADE was suppressed by more than 50% demonstrating a significant species bias to synthetic compared to organic waste-based media. The highest accumulation of protein and starch was exhibited in UTEX 1230 in ADE yielding 34% and 23% ash free dry weight (AFDW), respectively, though fatty acid methyl ester total lipid measured less than 12% AFDW. Results suggest that biomass from UTEX 1230 in ADE may serve as a candidate alga and growth system combination sustainable for animal feed production considering high yields of protein, starch and low lipid accumulation.