Early transcriptome responses of the bovine midcycle corpus luteum to prostaglandin F2α includes cytokine signaling

Authors

Heather Talbott, University of Nebraska Medical CenterFollow
Xiaoying Artegoitia, University of Nebraska Medical CenterFollow
Fang Qiu, University of Nebraska Medical CenterFollow
Pan Zhang, University of Nebraska Medical CenterFollow
Chittibabu Guda, University of Nebraska Medical CenterFollow
Fang Yu, University of Nebraska Medical CenterFollow
Robert A. Cushman, USDA-ARSFollow
Jennifer R. Wood, University of Nebraska-LincolnFollow
Cheng Wang, University of Nebraska Medical CenterFollow
Andrea S. Cupp, University of Nebraska-LincolnFollow
John S. Davis, University of Nebraska Medical CenterFollow

Document Type

Article

Date of this Version

2017

Citation

Molecular and Cellular Endocrinology 452 (2017) 93-109.

Comments

U.S. government work.

Abstract

In ruminants, prostaglandin F2alpha (PGF2α)-mediated luteolysis is essential prior to estrous cycle resumption, and is a target for improving fertility. To deduce early PGF2α-provoked changes in the corpus luteum a short time-course (0.5-4 h) was performed on cows at midcycle. A microarraydetermined transcriptome was established and examined by bioinformatic pathway analysis. Classic PGF2α effects were evident by changes in early response genes (FOS, JUN, ATF3) and prediction of active pathways (PKC, MAPK). Several cytokine transcripts were elevated and NF-kB and STAT activation were predicted by pathway analysis. Self-organizing map analysis grouped differentially expressed transcripts into ten mRNA expression patterns indicative of temporal signaling cascades. Comparison with two analogous datasets revealed a conserved group of 124 transcripts similarly altered by PGF2α treatment, which both, directly and indirectly, indicated cytokine activation. Elevated levels of cytokine transcripts after PGF2α and predicted activation of cytokine pathways implicate inflammatory reactions early in PGF2α-mediated luteolysis.