Date of this Version
Molecular and Cellular Endocrinology 452 (2017) 93-109.
In ruminants, prostaglandin F2alpha (PGF2α)-mediated luteolysis is essential prior to estrous cycle resumption, and is a target for improving fertility. To deduce early PGF2α-provoked changes in the corpus luteum a short time-course (0.5-4 h) was performed on cows at midcycle. A microarraydetermined transcriptome was established and examined by bioinformatic pathway analysis. Classic PGF2α effects were evident by changes in early response genes (FOS, JUN, ATF3) and prediction of active pathways (PKC, MAPK). Several cytokine transcripts were elevated and NF-kB and STAT activation were predicted by pathway analysis. Self-organizing map analysis grouped differentially expressed transcripts into ten mRNA expression patterns indicative of temporal signaling cascades. Comparison with two analogous datasets revealed a conserved group of 124 transcripts similarly altered by PGF2α treatment, which both, directly and indirectly, indicated cytokine activation. Elevated levels of cytokine transcripts after PGF2α and predicted activation of cytokine pathways implicate inflammatory reactions early in PGF2α-mediated luteolysis.
Cupp MCE 2017 Early transcriptome responses--suppl2 cupp.pdf (1010 kB)
Cupp MCE 2017 Early transcriptome responses--suppl3 cupp.xlsx (32 kB)
Cupp MCE 2017 Early transcriptome responses--suppl4 cupp.xlsx (254 kB)
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Cupp MCE 2017 Early transcriptome responses--suppl6 cupp.xlsx (37 kB)