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Developing heifers were offered either a modified distillers (MOD), distillers based (DDG), or corn gluten feed based (CGF) supplement while grazing pastures during development. Prior to breeding, antral follicle count (AFC), uterine horn diameter (UHD), ovarian area, and reproductive tract score (RTS) were determined via rectal ultrasonography to examine the effect of protein supplement on heifer reproductive characteristics. Heifers developed on MOD diets had greater RTS, ovarian area, and total AFC compared to DDG and CGF heifers. Small and medium follicle counts had a positive correlation with total AFC. Heifers developed on DDG and CGF had greater overall pregnancy rates compared to MOD heifers. We also conclude that there is a positive relationship between AFC and small and medium follicle counts.