Cytosolic Fe-S Cluster Protein Maturation and Iron Regulation Are Independent of the Mitochondrial Erv1/Mia40 Import System

Authors

• Hatice K. Ozer, University of South Carolina, Columbia
• Adrienne C. Dlouhy, University of South Carolina, Columbia
• Jeremy D. Thornton, Norwich Research Park, Norwich
• Jingjing Hu, University of South Carolina, Columbia
• Yilin Liu, University of Nebraska, Lincoln
• Joseph J. Barycki, University of Nebraska - LincolnFollow
• Janneke Balk, Norwich Research Park, Norwich
• Caryn E. Outten, University of South Carolina, Columbia

Document Type

Article

Date of this Version

2015

Citation

THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 290, NO. 46, pp. 27829–27840 , November 13, 2015

Comments

© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Abstract

Background: The mitochondrial sulfhydryl oxidase Erv1 is implicated in cytosolic iron-sulfur protein maturation and iron regulation.

Results: An erv1 yeast strain used in previous iron metabolism studies is glutathione-deficient, and erv1/mia40 mutants with sufficient glutathione do not exhibit iron-related defects.

Conclusion: Iron homeostasis is independent of Erv1/Mia40 function. Significance: The unexplained role of Erv1 in yeast iron metabolism is resolved.