EXOPEPTIDASE CATALYZED SITE-SPECIFIC BONDING OF SUPPORTS, LABELS AND BIOACTIVE AGENTS TO PROTEINS

Authors

Fred W. Wagner, Walton, Nebr.
Thomas R. Coolidge, Falls Village, Conn.
Dwane E. Wylie, Lincoln, Nebr.
Sheldon M. Schuster, Gainesville, Fla.
William Lewis, Lincoln, Nebr.
Jay Stout, Lincoln, Nebr.

Date of this Version

1994

Citation

Patent Number: 5,279,954

Comments

Appl. No.: 375,138

Abstract

The invention provides a means for attaching a label, support or bioactive agent to a protein with an exopeptidase at a site that is remote from the active site of the protein. More specifically the invention is directed to a method for the attachment of an amino acid, amine and alcohol nucleophile to the carboxyl terminus of a protein. In one embodiment, a labeled nucleophile is attached to a protein such as an antibody. In other embodiments, the invention is directed to a method for the attachment of a protein to an immobilization support and to a method for the attachment of a bioactive agent to a protein.