Genome-wide investigation of SnRK2 gene family in two jute species: Corchorus olitorius and Corchorus capsularis

Authors

Borhan Ahmed, Bangladesh Jute Research Institute
Fakhrul Hasan, Bangabandhu Sheikh Mujibur Rahman Agricultural University
Anika Tabassum, American International University of Bangladesh
Rasel Ahmed, Bangladesh Jute Research Institute
Rajnee Hassan, University of Nebraska-Lincoln
Ruhul Amin, Bangladesh Jute Research Institute
Mobashwer Alam, The University of Queensland

Document Type

Article

Date of this Version

2023

Citation

Ahmed et al. Journal of Genetic Engineering and Biotechnology (2023) 21:5 https://doi.org/10.1186/s43141-022-00453-x

Comments

Open access.

Abstract

Background Sucrose non-fermenting-1 (SNF1)-related protein kinase 2 (SnRK2), a plant-specifc serine/threonine kinase family, is associated with metabolic responses, including abscisic acid signaling under biotic and abiotic stresses. So far, no information on a genome-wide investigation and stress-mediated expression profling of jute SnRK2 is available. Recent whole-genome sequencing of two Corchorus species prompted to identify and characterize this SnRK2 gene family.

Result We identifed seven SnRK2 genes of each of Corchorus olitorius (Co) and C. capsularis (Cc) genomes, with similar physico-molecular properties and sub-group patterns of other models and related crops. In both species, the SnRK2 gene family showed an evolutionarily distinct trend. Highly variable C-terminal and conserved N-terminal regions were observed. Co- and CcSnRK2.3, Co- and CcSnRk2.5, Co- and CcSnRk2.7, and Co- and CcSnRK2.8 were upregulated in response to drought and salinity stresses. In waterlogging conditions, Co- and CcSnRk2.6 and Co- and CcSnRK2.8 showed higher activity when exposed to hypoxic conditions. Expression analysis in diferent plant parts showed that SnRK2.5 in both Corchorus species is highly expressed in fber cells providing evidence of the role of fber formation.

Conclusion This is the frst comprehensive study of SnRK2 genes in both Corchorus species. All seven genes identifed in this study showed an almost similar pattern of gene structures and molecular properties. Gene expression patterns of these genes varied depending on the plant parts and in response to abiotic stresses.