High phosphate (up to 600 mM) induces pseudohyphal development in five wild type Candida albicans

Authors

Jacob M. Hornby, Lewis-Clark State CollegeFollow
Raluca Dumitru, University of Nebraska - Lincoln
Kenneth Nickerson, University of Nebraska-LincolnFollow

Date of this Version

2004

Document Type

Article

Citation

Published in Journal of Microbiological Methods 56 (2004) 119– 124.

DOI: doi:10.1016/j.mimet.2003.09.021

Comments

Copyright © 2003 Elsevier B.V. Used by permission.

Abstract

A method is described for the formation of nearly 100% pseudohyphae populations of wild-type Candida albicans A72. The method employs fungal growth at 37 °C (ca. 5×10⁶ cells/ml) in a glucose–proline–N-acetyl-glucosamine medium supplemented with up to 600 mM phosphate (KH₂PO₄/K₂HPO₄ 1:1) at pH 6.5. Four other strains of C. albicans (MEN, 10261, SG5314 and CAI-4) also formed pseudohyphae under these conditions, although the phosphate response profiles differed in the concentration required for each strain to form pseudohyphae.