A Pressure Injection Device for Inoculation of Maize with Bacterial Phytopathogens

Authors

Randall R. Carlson, University of Nebraska-LincolnFollow
A. K. Vidaver, University of Nebraska-LincolnFollow
David S. Wysong, University of Nebraska-Lincoln
J. H. Riesselman, University of Nebraska-Lincoln

Document Type

Article

Date of this Version

9-1979

Citation

Plant Disease Reporter (September 1979) 63(9): 736-738

Published by the United States Department of Agriculture, Agricultural Research Service

Comments

Public domain

Abstract

A pressure injection device (PID) was used to inoculate maize (Zea mays) with bacterial phytopathogens. The PID was evaluated for its speed, effectiveness, and precision relative to the needle-eye method of inoculation. Corynebacterium nebraskense, Erwinia stewartii, and Pseudomonas andropogonis were each used successfully as inocula to infect maize and produce symptoms typical of Goss's wilt, Stewart's wilt and bacterial stripe, respectively. Quantitative inoculation with the PID was faster and as statistically precise as using a calibrated needle-eye.