Food Science and Technology, Department of
Core for Applied Genomics and Ecology (CAGE)
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Date of this Version
9-16-2008
Abstract
In 2005, a breakthrough technology was Announced that provided a quantum leap In DNA sequencing capacity.
This technology differed from the Sanger method In many ways:
1. Eliminates cloning—completely circumvents cloning bias
2. Uses bead technology—DNA templates are attached to sepharose beads
3. Relies on pyrosequencing chemistry—synthesis is sequential not partial
4. Parallelizes the sequencing process—hundreds of thousands of beads can be sequenced in parallel
The bottom line: it makes production-scale sequencing available to the common man.
Comments
Presentation from the workshop: Next-gen Sequencing for Research Scientists
September 15-16, 2008 East Campus Union
Presented by: Department of Food Science & Technology, University of Nebraska–Lincoln http://cage.unl.edu