Next Generation DNA Sequencing and the Birth of a Golden Era for Microbial Ecology

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Date of this Version

9-16-2008

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Presentation from the workshop: Next-gen Sequencing for Research Scientists

September 15-16, 2008 East Campus Union

Presented by: Department of Food Science & Technology, University of Nebraska–Lincoln http://cage.unl.edu

Abstract

In 2005, a breakthrough technology was Announced that provided a quantum leap In DNA sequencing capacity.

This technology differed from the Sanger method In many ways:
1. Eliminates cloning—completely circumvents cloning bias
2. Uses bead technology—DNA templates are attached to sepharose beads
3. Relies on pyrosequencing chemistry—synthesis is sequential not partial
4. Parallelizes the sequencing process—hundreds of thousands of beads can be sequenced in parallel

The bottom line: it makes production-scale sequencing available to the common man.