Mehmet İnan
Department of Chemical and Biomolecular Engineering
College of Engineering
University of Nebraska–Lincoln
Lincoln, Nebraska, United States

Research Interests:

• Physiology and molecular biology of methylotrophic yeast, Pichia pastoris, which is utilized as a system for the production of heterologous proteins (for example, vaccines, peptide hormones, and industrial enzymes).

• The effects of by-products on the protein production in P. pastoris and catabolite repression of methanol regulated AOX1 promoter of P. pastoris.

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2007

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Identification and characterization of calcium and manganese transporting ATPase (PMR1) gene of Pichia pastoris, Michael P. Dux and Mehmet Inan

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Cell bank characterization and fermentation optimization for production of recombinant heavy chain C-terminal fragment of botulinum neurotoxin serotype E (rBoNTE(Hc): Antigen E) by Pichia pastoris, Jayanta Sinha, Mehmet Inan, Sarah Fanders, Shinichi Taoka, Mark Gouthro, Todd Swanson, Rick Barent, Ardis Barthuli, Bonnie M. Loveless, Leonard A. Smith, Theresa Smith, Ian Henderson, John Ross, and Michael M. Meagher

2005

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Purification and scale-up of a recombinant heavy chain fragment C of botulinum neurotoxin serotype E in Pichia pastoris GS115, Michael P. Dux, Rick Barent, Jayanta Sinha, Mark Gouthro, Todd Swanson, Ardis Barthuli, Mehmet Inan, John T. Ross, Leonard A. Smith, Theresa J. Smith, Robert Webb, Bonnie Loveless, Ian Henderson, and Michael M. Meagher

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Crystallization and preliminary X-ray analysis of Na-ASP-1, a multi-domain pathogenesis-related-1 protein from the human hookworm parasite Necator americanus, Dr.Mehmet Inan, Oluwatoyin A. Asojo, Alex Lucas, Rick Barent, Jicai Huang, Brad Plantz, Amber Swanson, Mark Gouthro, Michael M. Meagher, and Peter J. Hotez

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Enhancement of Protein Secretion in Pichia pastoris by Overexpression of Protein Disulfide Isomerase, Mehmet Inan, Dinesh Aryasomayajula, Jayanta Sinha, and Michael M. Meagher

Maximization of Production of Secreted Recombinant Proteins in Pichia pastoris Fed-Batch Fermentation, Wenhui Zhang, Jayanta Sinha, Leonard A. Smith, Mehmet Inan, and Michael M. Meagher

2004

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Causes of Proteolytic Degradation of Secreted Recombinant Proteins Produced in Methylotrophic Yeast Pichia pastoris: Case Study With Recombinant Ovine Interferon-T, Mehmet Inan, Michael M. Meagher, Bradley A. Plantz, and Jayanta Sinha

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Optimization of cell density and dilution rate in Pichia pastoris continuous fermentations for production of recombinant proteins, Mehmet Inan, Michael M. Meagher, Wenhui Zhang, and Chih Ping Liu

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Causes of Proteolytic Degradation of Secreted Recombinant Proteins Produced in ethylotrophic Yeast Pichia pastoris: Case Study With Recombinant Ovine Interferon-T, Jayanta Sinha, Bradley A. Plantz, Mehmet Inan, and Michael Meagher

2001

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Non-Repressing Carbon Sources for Alcohol Oxidase (AOXI) Promoter of Pichia pastoris, MEHMET INAN and MICHAEL M. MEAGHER

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The Effect of Ethanol and Acetate on Protein Expression in Pichia pastoris, MEHMET INAN and MICHAEL M. MEAGHER

2000

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Studies on the alcohol oxidase (AOX1) promoter of Pichia pastoris, Mehmet Inan

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Fermentation Strategies for Recombinant Protein Expression in the Methylotrophic Yeast Pichia pastoris, Wenhui Zhang, Mehmet Inan, and Michael M. Meagher