Expression artifact with retroviral vectors based on pBMN

Authors

Yoshitaka Fukunaga, University of Nebraska Medical Center, Omaha
Robert A. Svoboda, University of Nebraska Medical Center, Omaha
Ronald Cerny, University of Nebraska - LincolnFollow
Keith R. Johnson, University of Nebraska Medical Center, OmahaFollow
Margaret J. Wheelock, University of Nebraska Medical Center, OmahaFollow

Date of this Version

12-2009

Comments

Published in Analytical Biochemistry 395:1 (December 1, 2009), pp. 49-53; doi: 10.1016/j.ab.2009.07.014 Copyright © 2009 Elsevier Inc. Used by permission.

Abstract

While characterizing various splice forms of p120 catenin, we observed what appeared to be a novel posttranslational modification of p120, resulting in a higher molecular weight form that was dependent on the splicing pattern. Further investigation revealed the higher molecular weight form to be a fusion protein between sequences encoded by the retroviral vector and p120. We found that the publicly available sequence of the vector we used does not agree with the experimental sequence. We caution other investigators to be aware of this potential artifact.