Preparation of High-Capacity Supports Containing Protein G Immobilized To Porous Silica

Authors

Abby J. Jackson, University of Nebraska - LincolnFollow
Elizabeth M. Karle, University of Nebraska - Lincoln
David S. Hage, University of Nebraska - LincolnFollow

Date of this Version

11-15-2010

Citation

Published in final edited form as: Anal Biochem. 2010 November 15; 406(2): 235–237. doi:10.1016/j.ab.2010.07.004. Version presented here is from NIH PubMed Central

Comments

Copyright Elsevier Inc. Used by permission.

Abstract

This study examined the preparation of high-capacity silica supports containing immobilized protein G. A maximum content of 39 mg protein G/g silica was obtained when using 100 Å pore size silica, followed by 33 mg/g for 50 Å silica and 9.3–24 mg/g for 300–4000 Å silica. The surface coverage of protein G increased with pore size, with a maximum level of 0.037 μmol/m² being obtained for 4000 Å silica. These supports gave comparable apparent activities (i.e., 30– 47% binding to rabbit immunoglobulin G, or IgG), with the highest binding capacities (71–77 mg IgG/g silica) being obtained for 50–100 Å silica.