Department of Chemistry
704 Hamilton Hall
University of Nebraska-Lincoln
Lincoln, Nebraska 68588 United States
dhage1@unl.edu
Research Interests:
Analytical chemistry, bioanalytical chemistry, analytical separation methods, high-performance liquid chromatography, capillary electrophoresis, affinity-based separations, affinity mass spectrometry, monolithic supports and hybrid separation media, affinity microcolumns and miniaturized separation systems, separation-based studies of biological interactions, analytical methods for personalized medicine, chromatographic immunoassays, immobilization methods for biochemicals.
2021
Recent Advances in Supramolecular Affinity Separations: Affinity Chromatography and Related Methods, Ashley G. Woolfork, Sazia Iftekhar, Susan Ovbude, Kyungah Suh, Sadia Sharmeen, Isaac Kyei, Jacob Jones, and David S. Hage
2020
Development of an on-line immunoextraction/entrapment system for protein capture and use in drug binding studies by high-performance affinity chromatography, Elliott L. Rodriguez, Saumen Poddar, Meera Choksi, and David S. Hage
Clinical and pharmaceutical applications of affinity ligands in capillary electrophoresis: A review, Chenhua Zhang, Ashley G. Woolfork, Kyungah Suh, Susan Ovbude, Cong Bi, Marawan Elzoeiry, and David S. Hage
2019
An Overview of Capillary Electrophoresis (CE) in Clinical Analysis, David S. Hage
Optimization of Protein Entrapment in Affinity Microcolumns using Hydrazide-Activated Silica and Glycogen as a Capping Agent, John Vargas-Badilla, Saumen Poddar, Shiden Azaria, Chenhua Zhang, and David S. Hage
Glycoform Analysis of Alpha1-Acid Glycoprotein by Capillary Electrophoresis Using Electrophoretic Injection, Chenhua Zhang, William Clarke, and David S. Hage
Development and Evaluation of Silica-Based Lectin Microcolumns for Glycoform Analysis of Alpha1-Acid Glycoprotein, Chenhua Zhang and David S. Hage
Development of a Microcolumn One-Site Immunometric Assay for a Protein Biomarker: Analysis of Alpha1-Acid Glycoprotein in Serum, Chenhua Zhang, Shae Lott, William Clarke, and David S. Hage
2018
Characterization of Solution-Phase Drug-Protein Interactions by Ultrafast Affinity Extraction, Sandya Beeram, Xiwei Zheng, Kyungah Suh, and David S. Hage
2017
Nanomaterials as Stationary Phases and Supports in Liquid Chromatography: A Review, Sandya Beeram, Elliott Rodriguez, Suresh Doddavenkatanna, Zhao Li, Allegra Pekarek, Darin Peev, Kathryn Goerl, Gianfranco Trovato, Tino Hofmann, and David S. Hage
Nanomaterials as Stationary Phases and Supports in Liquid Chromatography: A Review, Sandya Beeram, Elliott Rodriguez, Suresh Doddavenkatanna, Zhao Li, Allegra Pekarek, Darin Peev, Kathryn Goerl, Gianfranco Trovato, Tino Hofmann, and David S. Hage
2016
Chromatographic Studies of Protein-Based Chiral Separations, Cong Bi, Xiwei Zheng, Shiden Azaria, Sandya Beeram, Zhao Li, and David S. Hage
Optimizing Sequence Coverage for a Moderate Mass Protein in Nano-Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry, Ryan E. Matsuda, Venkata Kolli, Megan Woods, Eric D. Dodds, and David S. Hage
Anisotropic contrast optical microscope, D. Peev, T. Hofmann, N. Kananizadeh, S. Beeram, E. Rodriguez, S. Wimer, K.B. Rodenhausen, C.M. Herzinger, T. Kasputis, E. Pfaunmiller, A. Nguyen, Rafal Korlacki, A. Pannier, Y. Li, E. Schubert, D. Hage, and M. Schubert
2015
Affinity Chromatography: A Historical Perspective, David S. Hage and Ryan E. Matsuda
Chromatographic immunoassays: strategies and recent developments in the analysis of drugs and biological agents, Ryan E. Matsuda, Elliott Rodriguez, Doddavenkatanna Suresh, and David S. Hage
2014
Analysis of biomolecular interactions using affinity microcolumns: A review, Xiwei Zheng, Zhao Li, Sandya Beeram, Maria Podariu, Ryan Matsuda, Erika L. Pfaunmiller, Christopher J. White II, NaTasha Carter, and David S. Hage
2013
Effects of Fatty Acids and Glycation on Drug Interactions with Human Serum Albumin, Jeanethe Anguizola, Sara B. G. Basiaga, and David S. Hage
Development of Affinity Microcolumns for Drug–Protein Binding Studies in Personalized Medicine: Interactions of Sulfonylurea Drugs with in vivo Glycated Human Serum Albumin, Jeanethe Anguizola, K. S. Joseph, Omar S. Barnaby, Ryan Matsuda, Guadalupe Alvarado, William Clarke, Ronald Cerny, and David S. Hage
Review: Glycation of human serum albumin, Jeanethe Anguizola, Ryan Matsuda, Omar S. Barnaby, K. S. Hoy, Chunling Wa, Erin DeBolt, Michelle Koke, and David S. Hage
Use of entrapment and high-performance affinity chromatography to compare the binding of drugs and site-specific probes with normal and glycated human serum albumin, Abby J. Jackson, Jeanethe Anguizola, Erika L. Pfaunmiller, and David S. Hage
Studies of metabolite–protein interactions: A review, Ryan Matsuda, Cong Bi, Jeanethe Anguizola, Matthew Sobansky, Elliott Rodriguez, John Vargas Badilla, Xiwei Zheng, Benjamin Hage, and David S. Hage
Affinity monolith chromatography: A review of principles and recent analytical applications, Erika L. Pfaunmiller, Marie Laura Paulemond, Courtney M. Dupper, and David S. Hage
Analysis of free fractions for chiral drugs using ultrafast extraction and multi-dimensional high-performance affinity chromatography, Xiwei Zheng, Michelle J. Yoo, and David S. Hage
2012
Pharmaceutical And Biomedical Applications Of Affinity Chromatography: Recent Trends And Developments, David S. Hage, Jeanethe A. Anguizola, Cong Bi, Rong Li, Ryan Matsuda, Efthimia Papastavros, John Vargas-Badilla, and Xiwei Zheng
Restricted Access Media and Methods for Making Restricted Access Media, David S. Hage, Chunling Wa, Abby Jackson, and Hai Xuan
Analysis Of Drug Interactions With Modified Proteins By High-Performance Affinity Chromatography: Binding Of Glibenclamide To Normal And Glycated Human Serum Albumin, Ryan Matsuda, Jeanethe Anguizola, K.S. Joseph, and David S. Hage
Optimization of human serum albumin monoliths for chiral separations and high-performance affinity chromatography, Erika L. Pfaunmiller, Mahli Hartmann, Courtney M. Dupper, Sony Soman, and David S. Hage
Biointeraction Analysis of Immobilized Antibodies and Related Agents by High-Performance Immunoaffinity Chromatography, Erika L. Pfaunmiller, Annette C Moser, and David S. Hage
Identification and Analysis of Stereoselective Drug Interactions with Low Density Lipoprotein by High-performance Affinity Chromatography, Matt Sobansky and David S. Hage
IDENTIFICATION AND ANALYSIS OF STEREOSELECTIVE DRUG INTERACTIONS WITH LOW DENSITY LIPOPROTEIN BY HIGHPERFORMANCE AFFINITY CHROMATOGRAPHY, Matthew R. Sobansky and David S. Hage
2011
COMPARISON OF MODIFICATION SITES FORMED ON HUMAN SERUM ALBUMIN AT VARIOUS STAGES OF GLYCATION, Omar S. Barnaby, Ronald Cerny, William Clarke, and David S. Hage
QUANTITATIVE ANALYSIS OF GLYCATION PATTERNS IN HUMAN SERUM ALBUMIN USING 16O/18O-LABELING AND MALDI-TOF MS, Omar S. Barnaby, Ronald Cerny, William Clarke, and David S. Hage
Bioanalysis Young Investigator Award – sponsored by Waters- Michelle J. Yoo, David S. Hage
Chromatographic analysis of drug interactions in the serum proteome, David S. Hage, Jeanethe A. Anguizola, Abby J. Jackson, Ryan Matsuda, Efthimia Papastavros, Erika Pfaunmiller, Zenghan Tong, John Vargas-Badilla, Michelle J. Yoo, and Xiwei Zheng
Characterization Of Drug Interactions With Serum Proteins by Using High-Performance Affinity Chromatography, David S. Hage, Jeanethe Anguizola, Omar S. Barnaby, Abby Jo Jackson, Michelle J. Yoo, Efthimia Papastavros, Erika Pfaunmiller, Matt Sobansky, and Zenghan Tong
Research in bioanalysis and separations at the University of Nebraska – Lincoln, David S. Hage, Eric D. Dodds, Liangcheng Du, and Robert Powers
BINDING OF TOLBUTAMIDE TO GLYCATED HUMAN SERUM ALBUMIN, K.S. Joseph, Jeanethe Anguizola, and David S. Hage
HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY AND THE ANALYSIS OF DRUG INTERACTIONS WITH MODIFIED PROTEINS: BINDING OF GLICLAZIDE WITH GLYCATED HUMAN SERUM ALBUMIN, Ryan Matsuda, Jeanethe Anguizola, K.S. Joseph, and David S. Hage
DEVELOPMENT OF A FLOW-BASED ULTRAFAST IMMUNOEXTRACTION AND REVERSE DISPLACEMENT IMMUNOASSAY: ANALYSIS OF FREE DRUG FRACTIONS, John E. Schiel, Zenghan Tong, Chainarong Sakulthaew, and David S. Hage
CHARACTERIZATION OF INTERACTION KINETICS BETWEEN CHIRAL SOLUTES AND HUMAN SERUM ALBUMIN BY USING HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY AND PEAK PROFILING, Zenghan Tong and David S. Hage
Detection of Heterogeneous Drug-Protein Binding By Frontal Analysis and High-Performance Affinity Chromatography, Zenghan Tong, K.S. Joseph, and David S. Hage
KINETIC STUDIES OF DRUG-PROTEIN INTERACTIONS BY USING PEAK PROFILING AND HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY: EXAMINATION OF MULTI-SITE INTERACTIONS OF DRUGS WITH HUMAN SERUM ALBUMIN COLUMNS, Zenghan Tong, John E. Schiel, Efthimia Papastavros, Corey M. Ohnmacht, Quentin R. Smith, and David S. Hage
Use of Peak Decay Analysis and Affinity Microcolumns Containing Silica Monoliths for Rapid Determination of Drug-Protein Dissociation Rates, Michelle J. Yoo and David S. Hage
2010
QUANTITATIVE ANALYSIS OF GLYCATION SITES ON HUMAN SERUM ALBUMIN USING 16O/18O-LABELING AND MATRIXASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY, Omar S. Barnaby, Chunling Wa, Ronald Cerny, William Clarke, and David S. Hage
Chromatographic Studies of Changes in Binding Of Sulfonylurea Drugs to Human Serum Albumin Due To Glycation and Fatty Acids, Sara B. G. Basiaga and David S. Hage
Analysis Of Drug Interactions With High Density Lipoprotein By High-Performance Affinity Chromatography, Sike Chen, Matthew R. Sobansky, and David S. Hage
Preparation of High-Capacity Supports Containing Protein G Immobilized To Porous Silica, Abby J. Jackson, Elizabeth M. Karle, and David S. Hage
Entrapment of Proteins in Glycogen-Capped And Hydrazide-Activated Supports, Abby J. Jackson, Hai Xuan, and David S. Hage
CHROMATOGRAPHIC ANALYSIS OF ACETOHEXAMIDE BINDING TO GLYCATED HUMAN SERUM ALBUMIN, K.S. Joseph, Jeanethe Anguizola, Abby J. Jackson, and David S. Hage
Characterization of the Binding Of Sulfonylurea Drugs to HSA by High-Performance Affinity Chromatography, K.S. Joseph and David S. Hage
The Effects of Glycation on the Binding of Human Serum Albumin to Warfarin and L-Tryptophan, K.S. Joseph and David S. Hage
Analysis Of Drug-Protein Binding By Ultrafast Affinity Chromatography Using Immobilized Human Serum Albumin, Rangan Mallik, Michelle J. Yoo, Chad J. Briscoe, and David S. Hage
Immunoaffinity chromatography: an introduction to applications and recent developments, Annette C Moser and David S. Hage
Biointeraction analysis by high-performance affinity chromatography: Kinetic studies of immobilized antibodies, Mary Anne Nelson, Annette C. Moser, and David S. Hage
Analysis of Lidocaine Interactions with Serum Proteins Using High-Performance Affinity Chromatography, Sony Soman, Michelle J. Yoo, Yoon Jeong Jang, and David S. Hage
Biointeraction Analysis of Carbamazepine Binding To Alpha 1-Acid Glycoprotein by High-Performance Affinitychromatography, Hai Xuan, K. S. Joseph, Chunling Wa, and David S. Hage
Evaluation of Affinity Microcolumns Containing Human Serum Albumin for Rapid Analysis of Drug-protein Binding, Michelle J. Yoo, John E. Schiel, and David S. Hage
2009
Non-Competitive Peak Decay Analysis Of Drugprotein Dissociation By High-Performance Affinity Chromatography, Jianzhong Chen, John E. Schiel, and David S. Hage
Evaluation of Indole-Based Probes for High throughput Screening Of Drug Binding To Human Serum Albumin: Analysis by High-Performance Affinity Chromatography, Mandi L. Conrad, Annette C Moser, and David S. Hage
Characterization of Drug-Protein Interactions In Blood Using High-Performance Affinity Chromatography, David S. Hage, Abby Jackson, Matt Sobansky, John E. Schiel, Michelle J. Yoo, and K. S. Joseph
Evaluation of Alternatives to Warfarin as Probes For Sudlow Site I of Human Serum Albumin Characterization by High-Performance Affinity Chromatography, K.S. Joseph, Annette C Moser, John E. Schiel, and David S. Hage
Kinetic Studies of Biological Interactions By Affinity Chromatography, John E. Schiel and David S. Hage
Measurement Of Drug-Protein Dissociation Rates By High-Performance Affinity Chromatography And Peak Profiling, John E Schiel, Corey M. Ohnmacht, and David S. Hage
Evaluation of silica monoliths in affinity microcolumns for high-throughput analysis of drug–protein interactions, Michelle J. Yoo and David S. Hage
Studies of Imipramine Binding To Human Serum Albumin By High-Performance Affinity Chromatography, Michelle J. Yoo, Quentin R. Smith, and David S. Hage
2008
Development of an Affinity Silica Monolith Containing Human Serum Albumin For Chiral Separations, Rangan Mallik and David S. Hage
Immobilization Of Alpha 1-Acid Glycoprotein For Chromatographic Studies Of Drug-Protein Binding Ii. Correction For Errors In Association Constant Measurements, Rangan Mallik, Hai Xuan, Georges Guiochon, and David S. Hage
Studies of Verapamil Binding to Human Serum Albumin By High-Performance Affinity Chromatography, Rangan Mallik, Michelle J. Yoo, Sike Chen, and David S. Hage
Capillary Electrophoresis-Based Immunoassays: Principles & Quantitative Applications, Annette C Moser and David S. Hage
Estimating Protein-Ligand Binding Affinity using High- Throughput Screening by NMR, Matthew D. Shortridge PhD, David S. Hage, Gerard S. Harbison, and Robert Powers
Determination of Free Catecholamines in Urine By Tandem Affinity/Ion-Pair Chromatography and Flow Injection Analysis, David H. Thomas, Jay D. Taylor, Omar S. Barnaby, and David S. Hage
2007
Development Of Sulfhydryl-Reactive Silica For Protein Immobilization In High-Performance Affinity Chromatography, Rangan Mallik, Chunling Wa, and David S. Hage
Development Of An Affinity Silica Monolith Containing α1- Acid Glycoprotein For Chiral Separations, Rangan Mallik, Hai Xuan, and David S. Hage
Environmental Analysis By On-Line Immunoextraction And Reversed-Phase Liquid Chromatography: Optimization Of The Immunoextraction/RPLC Interface, Mary Anne Nelson, Efthimia Papastavros, Maud Dodlinger, and David S. Hage
Characterization of Glycation Adducts On Human Serum Albumin by Matrix Assisted Laser Desorption/ Ionization Time-Of-Flight Mass Spectrometry, Chunling Wa, Ronald Cerny, William A. Clarke, and David S. Hage
2006
Quantitative Studies of Allosteric Effects By Biointeraction Chromatography: Analysis of Protein Binding For Low Solubility Drugs, Jianzhong Chen and David S. Hage
Chromatographic analysis of carbamazepine binding to human serum albumin: II. Comparison of the Schiff base and N-hydroxysuccinimide immobilization methods, Hee Seung Kim, Rangan Mallik, and David S. Hage
Stability of warfarin solutions for drug–protein binding measurements: Spectroscopic and chromatographic studies, Annette C. Moser, Charles A. Kingsbury, and David S. Hage
Studies by biointeraction chromatography of binding by phenytoin metabolites to human serum albumin, Corey M. Ohnmacht, Shirley Chen, Zenghan Tong, and David S. Hage
Analysis of Free Drug Fractions Using Near-Infrared Fluorescent Labels and an Ultrafast Immunoextraction/Displacement Assay, Corey M. Ohnmacht, John E. Schiel, and David S. Hage
Identification and Quantitative Studies of Protein Immobilization Sites by Stable Isotope Labeling And Mass Spectrometry, Chunling Wa, Ronald Cerny, and David S. Hage
2002
Loading Microcolumns for the Separation of Analytes from a Sample in the Millisecond Time Scale, David S. Hage and William A. Clarke
1999
Affinity Chromatography: A Review of Clinical Applications, David S. Hage
1992
Intact Parathyroid Hormone: Performance and Clinical Utility of an Automated Assay Based on High-Performance Immunoaffinity Chromatography and Chemiluminescence Detection, David S. Hage, Bob Taylor, and Pai C. Kao
Improved Recovery of a Radlolabeled Peptide with an Albumin-Treated Reversed-Phase HPLC Column, David S. Hage, Robert L. Taylor, and Pai C. Kao
1991
Use of Affinity Chromatography in Developing Acridinium Ester-Labeled Antibodies for an Immunometric Assay of Parathyrin, David S. Hage, Bob Taylor, Pat Schryver, and Pai C. Kao