Studies of metabolite–protein interactions: A review

Authors

Ryan Matsuda, University of Nebraska-LincolnFollow
Cong Bi, University of Nebraska–Lincoln
Jeanethe Anguizola, University of Nebraska–Lincoln
Matthew Sobansky, University of Nebraska–LincolnFollow
Elliott Rodriguez, University of Nebraska–Lincoln
John Vargas Badilla, University of Nebraska–LincolnFollow
Xiwei Zheng, University of Nebraska–LincolnFollow
Benjamin Hage, University of Nebraska–Lincoln
David S. Hage, University of Nebraska - LincolnFollow

Date of this Version

2013

Citation

Published in Journal of Chromatography B, 2013: doi: 10.1016/j.jchromb.2013.11.043

Comments

Copyright © 2013 Elsevier B.V. Used by permission.

Abstract

The study of metabolomics can provide valuable information about biochemical pathways and processes at the molecular level. There have been many reports that have examined the structure, identity and concentrations of metabolites in biological systems. However, the binding of metabolites with proteins is also of growing interest. This review examines past reports that have looked at the binding of various types of metabolites with proteins. An overview of the techniques that have been used to characterize and study metabolite–protein binding is first provided. This is followed by examples of studies that have investigated the binding of hormones, fatty acids, drugs or other xenobiotics, and their metabolites with transport proteins and receptors. These examples include reports that have considered the structure of the resulting solute–protein complexes, the nature of the binding sites, the strength of these interactions, the variations in these interactions with solute structure, and the kinetics of these reactions. The possible effects of metabolic diseases on these processes, including the impact of alterations in the structure and function of proteins, are also considered.