Recent Advances in Supramolecular Affinity Separations: Affinity Chromatography and Related Methods

Authors

Ashley G. Woolfork, University of Nebraska-Lincoln
Sazia Iftekhar, University of Nebraska-LincolnFollow
Susan Ovbude, University of Nebraska-Lincoln
Kyungah Suh, University of Nebraska-LincolnFollow
Sadia Sharmeen, University of Nebraska-LincolnFollow
Isaac Kyei, University of Nebraska-LincolnFollow
Jacob Jones, University of Nebraska-LincolnFollow
David S. Hage, University of Nebraska-LincolnFollow

Date of this Version

2021

Citation

Published in Advances in Chromatography, volume 58 (2021), pp 1-73.

DOI: 10.1201/9781003223405-1

Comments

Copyright © 2021 CRC/Routledge. Used by permission

Abstract

Contents

1 Introduction

2 Supports for Affinity Chromatography

 2.1 Natural Supports and Related Materials

 2.2 Inorganic Supports

 2.3 Synthetic Supports

 2.4 Magnetic Beads and Particles

 2.5 Smart Materials

 2.6 Nanomaterials

 2.7 Support Formats

3 Immobilization Methods

 3.1 Non-Covalent Immobilization

 3.2 Covalent Immobilization

4 Binding Agents Used in Affinity Chromatography

 4.1 Biological Agents as Affinity Ligands

4.1.1 Immunoaffinity Chromatography

4.1.2 Immunoglobulin-Binding Proteins

4.1.3 Lectins

4.1.4 Enzymes

4.1.5 Serum Proteins

4.1.6 Biotin, Avidin, and Streptavidin

4.1.7 Carbohydrates

4.1.8 Lipids

4.1.9 Nucleic Acids

 4.2 Non-Biological Agents as Affinity Ligands

4.2.1 Boronates and Related Mixed Ligands

4.2.2 Dye-Ligands

4.2.3 Immobilized Metal-Ion Chelates

4.2.4 Molecularly Imprinted Polymers

5 Applications of Affinity Chromatography

 5.1 Preparative Applications

5.1.1 Enzyme and Protein Purification

5.1.2 Purification of Viral Particles, Cells, and Related Targets

 5.2 Analytical Applications

 5.3 Chiral Separations

 5.4 Biointeraction Studies

5.4.1 Zonal Elution Methods

5.4.2 Frontal Analysis Methods

5.4.3 Methods for Kinetic Studies

6 Conclusions

Acknowledgments

References