Food Science and Technology, Department of
Department of Food Science and Technology: Faculty Publications
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Document Type
Article
Date of this Version
1-30-2014
Citation
Phanse, Y. et al. A systems approach to designing next generation vaccines: combining a-galactose modified antigens with nanoparticle platforms. Sci. Rep. 4, 3775; DOI:10.1038/srep03775 (2014).
Abstract
Innovative vaccine platforms are needed to develop effective countermeasures against emerging and re-emerging diseases. These platforms should direct antigen internalization by antigen presenting cells and promote immunogenic responses. This work describes an innovative systems approach combining two novel platforms, aGalactose (aGal)-modification of antigens and amphiphilic polyanhydride nanoparticles as vaccine delivery vehicles, to rationally design vaccine formulations. Regimens comprising soluble aGal-modified antigen and nanoparticle-encapsulated unmodified antigen induced a high titer, high avidity antibody response with broader epitope recognition of antigenic peptides than other regimen. Proliferation of antigen-specific CD4+ T cells was also enhanced compared to a traditional adjuvant. Combining the technology platforms and augmenting immune response studies with peptide arrays and informatics analysis provides a new paradigm for rational, systems-based design of next generation vaccine platforms against emerging and re-emerging pathogens.
Comments
This work is licensed under a Creative Commons Attribution- NonCommercial-NoDerivs 3.0 Unported license. Used by permission.