Ara h 6 Complements Ara h 2 as an Important Marker for IgE Reactivity to Peanut

Authors

• Audrey E. Koid, INDOOR Biotechnologies, Inc.
• Martin D. Chapman, INDOOR Biotechnologies, Inc.
• Robert G. Hamilton, Johns Hopkins University School of Medicine
• Ronald van Ree, Academic Medical Center
• Serge A. Versteeg, Academic Medical Center
• Stephen C. Dreskin, University of Colorado Denver
• Stef J. Koppelman, University of Nebraska-LincolnFollow
• Sabina Wünschmann, INDOOR Biotechnologies, Inc.

Document Type

Article

Date of this Version

1-8-2014

Citation

J Agric Food Chem. 2014 January 8; 62(1): 206–213. doi:10.1021/jf4022509.

Comments

Copyright 2014 American Chemical Society. Used by permission.

Abstract

The similarities of two major peanut allergens, Ara h 2 and Ara h 6, in molecular size, amino acid sequence, and structure have made it difficult to obtain natural Ara h 6 free of Ara h 2. The objectives of this study were to purify natural Ara h 6 that is essentially free of Ara h 2 and to compare its IgE reactivity and potency in histamine release assays to Ara h 2. SDS-PAGE of the highly purified allergen (<0.01% Ara h 2) revealed a single 14.5kD band and the identity of Ara h 6 was confirmed by LC-MS/MS. Ara h 6 showed a higher seroprevalence in chimeric-IgE ELISA (n=54), but a weaker biological activity in basophil histamine release assays than Ara h 2. Purified Ara h 6 will be useful for diagnostic IgE antibody assays, as well as molecular and cellular studies to investigate the immunological mechanisms of peanut allergy.