Influence of Foliar Fungicide Treatment on Lipolytic Enzyme Activity of Whole Wheat

Authors

Rachana Poudel, University of Nebraska - LincolnFollow
Madhav Bhatta, University of Nebraska - LincolnFollow
Teshome Regassa, University of Nebraska-LincolnFollow
Devin J. Rose, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

2017

Citation

Published in Cereal Chemistry 94:3 (2017), pp 633–639.

doi 10.1094/CCHEM-11-16-0279-R

Comments

Copyright © 2017 AACC International, Inc. Published by John Wiley Inc. Used by permission.

Abstract

Lipolytic enzymes play a key role in the deterioration of whole wheat flour upon storage but may also be involved in plant disease and stress tolerance while the crop is in the field. Therefore, the purpose of this study was to determine the effect of foliar fungicide treatment on lipolytic activity in wheat. A significant cultivar × fungicide × year interaction for esterase (p-nitrophenyl butyrate as substrate [EA-B]) and lipoxygenase (LOX) activities was observed; however, a large portion of the variability was owing to year (environment). Fungicide influenced lipase (olive oil as substrate [LA-O]), EA-B, and LOX activities. Lipase (p-nitrophenyl palmitate as substrate [LA-P]) showed variation in terms of cultivar and year rather than the application of fungicide. Partial correlation (year as a partial variable) between LA-P and EA-B activities was observed (r = 0.78, P < 0.001), although neither was correlated with LA-O. The influence of foliar fungicide on lipolytic enzyme activities depends mostly on growing conditions but is also affected by disease stress, disease resistance of the varieties tested, and the substrate being used in the assay.