Correlation of Periodontal Pathogens in Concurrent Endodontic-Periodontal Diseases

Authors

Amela Lačević, University of SarajevoFollow
Federico Foschi, King’s College London Dental Institute at Guy’s & Guy’s Hospital
Lejla Pojskić, University of Sarajevo
Naris Pojskić, University of Sarajevo
Kasim Bajrović, University of Sarajevo
Jacques Izard, Forsyth Institute and Harvard UniversityFollow

ORCID IDs

Izard https://orcid.org/0000-0002-5904-5436

Document Type

Article

Date of this Version

8-2015

Citation

Oral Biology and Dentistry (August 2015) 3: 5

doi: 10.7243/2053-5775-3-5

Comments

License: CC BY 3.0

Abstract

Objectives: This study investigated the correlation between Tannerella forsythia, Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans at dual sites in concurrent endodonticperiodontal diseases.

Material and methods: Samples were collected from endodontium and periodontium in cases of concurrent endodontic-periodontal diseases from thirty participants. The sensitivity and specificity of SYBR Green real-time PCR was used to identify the targeted species. Absolute number of targeted genome copies in tested samples were extrapolated from respective calibration curve.

Results: No statistical difference was found in the number of detected endodontic-periodontal pathogens between the endodontium and periodontium. The Pearson test detected significant correlation (P < 0.001) between targeted bacteria; T. forsythia, F. nucleatum, and P. gingivalis from endodontic-periodontal lesions. Synergistic component observed separately in endodontic biofilm was found only between T. forsythia and F. nucleatum (r = 0.380, P = 0.03) while in periodontal biofilm T. forsythia, F. nucleatum, and P. gingivalis gave high synergism result (P < 0.0001). Correlation analysis showed that T. forsythia in primary endodontic infection and in periodontal lesion was significantly decreased with the increase of patients age (r = -0.308, P = 0.017).

Conclusions: Correlation between targeted bacterial species levels from concurrent endodonticperiodontal diseases confirmed that coronal and cervical dentinal tubules may represent a viable pathway that allows spreading and maintaining of dual sites infection. Periodontal bacteria detected in root canal of concurrent endodontic-periodontal infections may originate from the local periodontal lesions.