Evaluation and Comparison of the Species-Specificity of 3 Antiparvalbumin IgG Antibodies

Authors

Poi-Wah Lee, University of Nebraska-Lincoln
Julie A. Nordlee, University of Nebraska-LincolnFollow
Stef J. Koppelman, University of Nebraska-Lincoln and HAL Allergy, Leiden, NetherlandsFollow
Joseph L. Baumert, University of Nebraska-LincolnFollow
Steve L. Taylor, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

2011

Citation

Journal of Agricultural and Food Chemistry (2011) 59(23): 12,309–12,316

doi: 10.1021/jf203277z

Comments

Copyright © 2011, American Chemical Society. Used by permission

Abstract

Parvalbumin is a pan-allergen in fish and frogs that triggers IgE-mediated reactions in fish-allergic individuals. Previous studies demonstrated that antibodies raised against fish and frog parvalbumins displayed varying specificity for different fish species, and thus, the applicability of these antibodies for potential use in immunoassays to detect fish residues were limited. We aimed to determine the specificity of 3 IgG antibodies for various fish species. Indirect enzyme-linked immunosorbent assay (ELISA) and IgG-immunoblotting were used to compare the reactivity of the polyclonal anticod parvalbumin antibody and the commercially available, monoclonal antifrog and monoclonal anticarp parvalbumin antibodies against raw muscle extracts of 29 fish species. All antibodies demonstrated varying specificities for different fish species. Of the 3 antibodies, the polyclonal anticod parvalbumin antibody is the most suitable for the detection of fish parvalbumins, as it showed reactivity to the widest range of species, including herring, pilchard, carp, pike, cod, pollock, haddock, cusk, hake, bluegill, tilapia, bass, grouper, trout, catfish, and perch, although detection was still limited for several key fish species.