Nutrition and Health Sciences, Department of
First Advisor
Ivan Vechetti
Date of this Version
4-2023
Citation
Kerr et al
Abstract
We sought to ascertain the effect of different types of exercise (aerobic training and resistance training) on the lipid profile of extracellular vesicles. Thus, we had participants perform bouts of AT and RT and rest (control) and collected blood samples from them immediately after (timepoint 0) and 30 minutes after (timepoint 30) exercise. Small extracellular vesicles (sEVs) were isolated from plasma of participants using cushioned-density gradient ultracentrifugation (C-DGUC). Small EV size, morphology, and protein markers were determined using nanoparticle tracking analysis, transmission electron microscopy, and western blot, respectively. Additionally, we performed untargeted lipidomics on the sEV isolate. We found that RT significantly increases the size of sEVs immediately after exercise and that both types of exercise causes slight changes in the lipid profile of circulating sEVS. We specifically observed changes in Anandamide (AEA), Ceramide (Cer), Cholesterol Ester (ChE), Carboxymethyl ethanolamine (CmE), Trihexosylceramide (Hex3Cer), Lyso- phosphatidylglycerol (LPG) Lyso-phosphatidylinositol (LPI), Lyso-sphingomyelin (LSM), and Wax monoesters (WE).
Advisor: Ivan Vechetti
Included in
Biochemical Phenomena, Metabolism, and Nutrition Commons, Physiological Processes Commons
Comments
A THESIS Presented to the Faculty of The Graduate College at the University of Nebraska In partial Fulfillment of Requirement For the Degree of Master of Science, Major: Nutrition and Health Sciences, Under the Supervision of Professor Ivan Vechetti. Lincoln, Nebraska: April, 2023
Copyright © 2023 Tesha Kerr