Agronomy and Horticulture Department
Protein Detection in Plants
Date of this Version
2005
Document Type
Article
Citation
Plant and Soil Sciences eLibrary (PASSeL) Lesson
This lesson has been assigned Journal Series No. 1018, Univ of Nebraska Cooperative Extension
Abstract
This lesson will focus on molecular principles involved in the detection of biotechnology derived proteins in crops, using the lateral flow ELISA.
Overview
There are instances in which information regarding the presence and/or amount of a particular protein made by a plant, animal, or human is needed. Fortunately there are laboratory methods available for making these measurements. Some of these methods are more extensive, while others are quicker and easier to carry out. This lesson will focus on molecular principles involved in the detection of biotechnology derived proteins in crops, using the lateral flow ELISA.
Objectives
This lesson builds upon gene expression principles discussed in the online lesson Gene Expression Part 1: Reading Genes to Make Proteins and the animation Protein Synthesis.
At the completion of this Protein Detection in Plants lesson, learners will be able to:
- List examples of when protein detection is required.
- Describe information needed to make an accurate sample to detect protein.
- Identify the role of antibodies in immunoassays.
- Explain the principles and steps of a lateral flow strip test.
- Outline the strengths and weaknesses of immunoassays.
Modules:
Comments
Copyright © 2005 Deana Namuth. Used by permission.
JNRLSE approved 2005
This project was supported in part by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, administered by the University of California-Davis and by the National Science Foundation (NSF), Division of Undergraduate Education, National SMETE Digital Library Program, Award #0938034, administered by the University of Nebraska. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA or NSF.
Special thanks to David Grothaus and Gary Thull (Pioneer) and James Stave (SDI) for their assistance and use of graphics/animations. The author also wishes to acknowledge the following companies and organizations for their contributions to this lesson: University of Nebraska–Lincoln, Department of Agronomy and Horticulture, AEIC, EnviroLogix, GeneScan, Medallion Labs, Monsanto, SDI, USFDA, and Pioneer.