United States Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
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Document Type
Article
Date of this Version
2012
Citation
Journal of Veterinary Diagnostic Investigation 24, 2012
Abstract
Pseudorabies virus (PRV) is the cause of Aujeszky’s disease, a disease that is significant economically for the swine industry worldwide. A real-time polymerase chain reaction (PCR) assay based on the gB and gE genes was used to identify PRV nucleic acid in diagnostic samples. Using virus isolation (VI) as the gold standard, the PCR assay performed well in a variety of diagnostic matrices. Testing was conducted on 1,027 nasal swabs with the following findings: gB sensitivity: 94.6% (95% confidence interval [CI]: 92.3–96.4%), specificity: 71.0% (95% CI: 64.0–77.3%); gE sensitivity: 94.6% (95% CI: 92.3–96.4%), specificity: 79.3% (95% CI: 72.9–84.7%). Diagnostic performance of the real-time PCR assay developed as a testing method indicates that it is a rapid, accurate assay that can provide reliable results on clinical samples.
Comments
U.S. Government