FUM9 Is Required for C-5 Hydroxylation of Fumonisins and Complements the Meitotically Defined Fum3 Locus in Gibberella moniliformis

Authors

Robert A. E. Butchko, USDA-ARSFollow
Ronald D. Plattner, USDA-ARS
Robert H. Proctor, USDA-ARSFollow

Date of this Version

11-2003

Citation

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 69, No. 11 (Nov. 2003), pp. 6935–6937. DOI: 10.1128/AEM.69.11.6935–6937.2003.

Comments

U.S. government work.

Abstract

Deletion of the Gibberella moniliformis FUM9 gene resulted in mutants that produce only fumonisins that lack a C-5 hydroxyl group. This phenotype is identical to that of previously described mutants with defective alleles at the meiotically defined Fum3 locus. Transformation with a wild-type FUM9 gene into a Fum3- defective mutant restored wild-type fumonisin production. These results indicate that the FUM9 protein catalyzes the C-5 hydroxylation of fumonisins and that FUM9 and the Fum3 locus are the same gene.