U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska
Document Type
Article
Date of this Version
3-2017
Citation
ANNUAL REPORT OF THE BEAN IMPROVEMENT COOPERATIVE, No. 60, March 2017. Published by USDA.
Abstract
INTRODUCTION The genus Phaseolus is attacked by soil fungus, Fusarium oxysporum f. sp. Phaseol, which causes wilt or yellowing (Nunes, 2003), and once introduced to crops, has its most viable and effective control with the use of resistant cultivars (Sartorato & Rava, 1994). Considering that obtaining disease-resistant cultivars is one of the most efficient ways to avoid considerable losses of productivity (Pereira & Ribeiro, 2012), this work aimed to evaluate the performance of snap bean of determined growth as resistance to Fusarium Oxysporum f. sp. Phaseoli during the development of plants.
MATERIAL AND METHODS Four strains of bean pods of determined growth habit were evaluated, using as a control the cultivar UEL 1. The sowing occurred on 04/03/2011, with five seeds per pot. For sowing, ceramic vessels with a capacity of five liters were used, with spacing of 0.1 m x 0.6 m between vessels. The soil used consisted of a clay soil with a clay texture, corrected through the formulated fertilizer 08-28-16 at the dose of 0.05 kg per pot. After the emergency, thinning was performed, maintaining one plant per pot. In the preparation of the inoculum the modified methodology of inoculation with colonized sorghum grains was used (Gasperi, 2000; Klingelfuss et al., 2002). Following the methodology, maize grains colonized with Fusarium were used for inoculation of the bean-pod strains, performed on 01/04/2011, when the plant reached the V4 development stage. In this procedure, two colonies of corn colonized on the substrate of each vessel were introduced. At the end of the crop cycle the yellowing symptoms caused by Fusarium were evaluated, and there were no lesions in the plant colon. In this evaluation, grades were assigned according to Gasperi's methodology (2000): resistant 0-1,0; Moderately resistant 1.1-1.5; Moderately susceptible 1.6-2.0; Susceptible 2.1-2.5 and highly susceptible 2.6-3.0. In the harvest performed on 06/05/2011, pods, roots and aerial part of the plants were weighed. The experimental design was completely randomized, in a 5x2 factorial scheme (five lines and two treatments, inoculated with Fusarium and without inoculation), with four replications. The data were submitted to analysis of variance and the means of the treatments compared by the Tukey test at 5% probability.
Comments
U.S. government work.