U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska
Document Type
Article
Date of this Version
3-2017
Citation
ANNUAL REPORT OF THE BEAN IMPROVEMENT COOPERATIVE, No. 60, March 2017. Published by USDA.
Abstract
Powdery mildew (PM) is being one of the most serious diseases affecting bean crops in the north of Spain. Two independent resistance genes were reported in the cultivar Porrillo Sintetico, one of them located on linkage group (LG) Pv11, conferring partial resistance, and other located on LG Pv04 conferring a total resistance response (Campa and Ferreira 2017). The objective of this work was the introgression of the gene conferring total resistance from Porrillo Sintetico into the line X2776. Genomic regions introgressed after the breeding program were studied using ‘genotyping by sequencing’ (GBS).
MATERIAL AND METHODS X2776 is a breeding line developed at the SERIDA having fabada seed phenotype (white and very large seeds), determinate growth habit (finfin) and two introgressed resistance locus against virus (I gene) and anthracnose (Co-2 cluster) (Ferreira et al. 2016). The X2776 line shows a partial resistance response against PM.
Total resistance in X2776 was introgressed using a backcrossing method including six backcross generations followed by four self-pollinated generations with individual plant selection using resistance tests. Seed and plant phenotype were also considered in the selection process of each generation. Resistance tests were carried out according to Trabanco et al. (2012) using a local isolate of PM maintained on plants of susceptible bean cv. Xana in spore-proof chambers.
The parental lines X2776, Porrillo Sintetico and four resistant plants derived from F4BC6 families were genotyped through GBS using the ApeKI restriction enzyme. Physical positions of the SNP markers were established using the Phaseolus vulgaris genome v1 (GenBank Assembly accession GCF_000499855.1).
Comments
U.S. government work.