U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska
Document Type
Article
Date of this Version
3-2017
Citation
ANNUAL REPORT OF THE BEAN IMPROVEMENT COOPERATIVE, No. 60, March 2017. Published by USDA.
Abstract
INTRODUCTION: A whole spectrum of soil microorganisms in the rhizosphere, may affect the bean (Phaseolus vulgaris L.) yield as well as the interaction among the different microorganisms which might exist in the soil. The bean plant has also the opportunity to interact with other microorganisms as in the case of the bacteria (Gherbi et al., 2008; Marsh et al., 2008; Oldroyd et al., 2009). The nitrogen fixing bacteria such as a strain of Rhizobium tropici CIAT 899, are capable of fixing the atmospheric nitrogen when it occurs in symbiosis with its host plant, in this case the bean plant.
MATERIALS AND METHODS: The experiment was carried out in a greenhouse of Colegio de Postgraduados at Montecillo, state of Mexico in July of 2016. Two cultivars of determinate growth habit were employed: OTI and Cacahuate 72. A group of seedlings in the simple leaf stage were inoculated with a suspension of strain Rhizobium tropici CIAT 899 bacteria. Another group was not inoculated. The plants were grown in plastic containers with 7 kg of sandy loam soil. The experimental design was a complete randomized with five replications. The following treatments were evaluated: 1) cv. OTI inoculated; 2) cv. OTI non inoculated; 3) cv. Cacahuate 72 inoculated and 4) cv. Cacahuate 72 non inoculated. Sixty nine days after planting, the rates of photosynthesis and transpiration were determined. At physiological maturity there were registered: the number of normal pods, seeds and seed yield (all per plant). The statistical analysis was performed with the SAS program (SAS, 2012).
Comments
U.S. government work.