U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska
Date of this Version
3-2017
Citation
ANNUAL REPORT OF THE BEAN IMPROVEMENT COOPERATIVE, No. 60, March 2017. Published by USDA.
Abstract
INTRODUCTION The insight into the complex processes of biological systems encoded by the plant and animal genome can be focused by studying the network of gene products (Pandy and Mann, 200) which are resulted from gene expression determined by the complex interactions among transcription factors, chromatin proteins, and epigenetic modifications. The long-term goals of our research are to understand the transcriptomic and epigenetic components involved in the translocation of health related micronutrients in common bean. Previously, we identified a common bean genotype highly responsive to higher concentration of Fe (Bauduin et al. 2014). In this work, we applied higher concentrations of Fe to a responsive common bean genotype and analyzed isolated proteins to identify differences in protein expression between treatments using SDS-PAGE and mass spectrometry.
MATERIALS AND METHODS At Mayville State University, we grew three replications of the bean genotype (G122) with controls. We planted the seeds in 8.5’’x11’’ pots filled with “Sunshine Mix”. The sunshine mix was soaked with water until germination. After germination, we kept filling the plastic saucer beneath the treated plants’ pot with solutions of 150mg-1L and (200mg-1L) Fe until 50% leaf senescence, while the controls continued receiving water. After harvesting, seed samples were sent to a Proteomics laboratory at University Maryland College Park for SDS-PAGE and Mass Spectrometry analysis.
Comments
U.S. government work.