Gypenosides Reduced the Risk of Overweight and Insulin Resistance in C57BL/6J Mice through Modulating Adipose Thermogenesis and Gut Microbiota

Authors

Jie Liu, Shanghai Jiao Tong University
Yanfang Li, Shanghai Jiao Tong University
Puyu Yang, Shanghai Jiao Tong University
Jianchun Wan, Shanghai Jiao Tong University
Qimeng Chang, Fudan University Minhang Hospital
Thomas T. Y. Wang, USDA-ARSFollow
Weiying Lu, Shanghai Jiao Tong University
Yaqiong Zhang, Shanghai Jiao Tong University
Qin Wang, University of Maryland
Liangli Lucy Yu, University of MarylandFollow

Document Type

Article

Date of this Version

2017

Citation

J. Agric. Food Chem. XXXX, XXX, XXX-XXX

Comments

U.S. government work.

Abstract

This study investigated whether and how gypenosides from jiaogulan tea at 100 and 300 mg/kg/day levels could reduce the development of overweight and insulin resistance in C57 BL/6J mice fed a high-fat diet in 12 weeks. The 300 mg/kg/ day gypenosides supplement significantly reduced final body weight, plasma total cholesterol, and homeostasis model assessment-estimated insulin resistance (HOMA-IR) index by 19.9%, 40%, and 36%, respectively, compared with the high-fat diet control group. Gypenosides also increased brown adipocyte tissue activity and white adipose tissue browning. The expression of genes involved in mitochondrial activity and fatty acid β-oxidation were also increased in both brown and white adipocyte tissues. In addition, gypenosides at 100 and 300 mg/kg/day levels decreased the ratio of Firmicutes to Bacteroidetes by 20% and 58.6%, respectively, and increased Akkermansia muciniphila abundance in the gut microbiota.