Murine Monoclonal Antibodies Specific for Lipopolysaccharide of Escherichia coli O26 and O111

Authors

Mildred Rivera-Betancourt, Roman L. Hruska U.S. Meat Animal Research Center, USDA-ARS, Clay Center, Nebraska
James E. Keen, Roman L. Hruska U.S. Meat Animal Research Center, USDA-ARS, Clay Center, NebraskaFollow

Date of this Version

9-1-2000

Comments

Published in APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept. 2000, p. 4124–4127.

Abstract

Monoclonal antibody (MAb) 12F5 reacted with 35 Escherichia coli O26 isolates and cross-reacted with 1 of 365 non-E. coli O26 isolates. MAb 15C4 reacted with 30 E. coli O111 strains and 8 Salmonella O35 strains (possessing identical O antigen) but not with 362 other bacterial strains. Lipopolysaccharide immunoblots confirmed MAb O-antigen specificity.