Murine Monoclonal Antibodies against Escherichia coli O4 Lipopolysaccharide and H5 Flagellin

Authors

Mildred Rivera-Betancourt, United States Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center (MARC), Clay Center, Nebraska
James E. Keen, United States Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center (MARC), Clay Center, NebraskaFollow

Date of this Version

9-1-2001

Comments

Published in JOURNAL OF CLINICAL MICROBIOLOGY, Sept. 2001, p. 3409–3413.

Abstract

Two murine monoclonal antibodies (MAb), 2C5-F10 and 8D1-H10, reactive with Escherichia coli O4 and H5 antigens, respectively, were generated and characterized. Enzyme immunoassays and immunoblots demonstrated that MAb 2C5-F10 reacted specifically with lipopolysaccharide O antigen of E. coli O4 isolates, while MAb 8D1-H10 reacted with E. coli strains expressing H5 flagella.