Genetic analysis of Trichinella populations by ‘cold’ single-strand conformation polymorphism analysis

Authors

Robin B. Gasser, The University of Melbourne & Macquarie UniversityFollow
Min Hu, The University of Melbourne
Youssef Abs EL-Osta, The University of Melbourne
Dante S. Zarlenga, US Department of Agriculture, ARS, Bovine Functional Genomics Laboratory
Edoardo Pozio, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita

Document Type

Article

Date of this Version

2005

Citation

U.S. Government Works

Comments

Veterinary Parasitology 132 (2005) 23–26

doi:10.1016/j.vetpar.2005.05.021

Abstract

A non-isotopic single-strand conformation polymorphism (‘cold’ SSCP) technique has been assessed for the analysis of sequence variability in the expansion segment 5 (ES5) of domain IV and the D3 domain of nuclear ribosomal DNA within and/or among isolates and individual muscle (first-stage) larvae representing all currently recognized species/genotypes of Trichinella. Data are consistent with the ability of cold SSCP to identify intra-specific as well as inter-specific variability among Trichinella genotypes. The cold SSCP approach should be applicable to a range of other genetic markers for comparative studies of Trichinella populations globally.