Genetic analysis of Trichinella populations by ‘cold’ single-strand conformation polymorphism analysis

Authors

• Robin B. Gasser, The University of Melbourne & Macquarie UniversityFollow
• Min Hu, The University of Melbourne
• Youssef Abs EL-Osta, The University of Melbourne
• Dante S. Zarlenga, US Department of Agriculture, ARS, Bovine Functional Genomics Laboratory
• Edoardo Pozio, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita

Document Type

Article

Date of this Version

2005

Citation

U.S. Government Works

Comments

Veterinary Parasitology 132 (2005) 23–26

doi:10.1016/j.vetpar.2005.05.021

Abstract

A non-isotopic single-strand conformation polymorphism (‘cold’ SSCP) technique has been assessed for the analysis of sequence variability in the expansion segment 5 (ES5) of domain IV and the D3 domain of nuclear ribosomal DNA within and/or among isolates and individual muscle (first-stage) larvae representing all currently recognized species/genotypes of Trichinella. Data are consistent with the ability of cold SSCP to identify intra-specific as well as inter-specific variability among Trichinella genotypes. The cold SSCP approach should be applicable to a range of other genetic markers for comparative studies of Trichinella populations globally.