Lymphoproliferative and Gamma Interferon Responses to Stress- Regulated Mycobacterium avium subsp. Gurungparatuberculosis Recombinant Proteins

Authors

Ratna B. Gurung, University of Sydney, CamdenFollow
Douglas J. Begg, University of Sydney
Auriol C. Purdie, University of Sydney
Kumudika de Silva, University of Sydney, Camden
John Bannantine, USDA ARS National Animal Disease CenterFollow
Richard J. Whittington, University of Sydney

Document Type

Article

Date of this Version

2014

Citation

Clinical and Vaccine Immunology Volume 21 Number 6 p. 831–837

doi:10.1128/CVI.00775-13

Comments

2014, American Society for Microbiology

Abstract

Johne’s disease in ruminants is a chronic infection of the intestines caused by Mycobacterium avium subsp. paratuberculosis. An important strategy to control disease is early detection, and a potentially efficient method for early detection is measurement of cell-mediated immune responses developed by the host in response to exposure or infection. One method is to measure lym- phoproliferation and cytokine release from the host cells when exposed to the organism or parts of the organism. In this study, 10 recombinant M. avium subsp. paratuberculosis proteins known to be upregulated under in vitro stress conditions were evalu- ated by examining their ability to evoke memory as a result of exposure by vaccination or oral challenge with live Mycobacterium avium subsp. paratuberculosis. Out of 10 proteins, MAP2698c was found to induce higher cell-mediated immune responses in vaccinated and challenged sheep in comparison to healthy controls. The findings suggest that not all stress-regulated proteins have the diagnostic potential to detect cell-mediated immune responses in ovine paratuberculosis.